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This article represents the results of studies of the microorganism resistance to antimicrobial agents detected by PCR in the milk of cows and goats. In 94.1% of cow milk and in 80.7% of goat milk samples specific DNA regions of microorganisms were detected: Staphylococcus spp., E. coli, S. aureus, S. agalactiae. The more common pathogenic agents were the bacteria of the Staphylococcus spp. group (S. epidermidis, S. saprophyticum, S. haemolyticus) and E. coli. The opportunistic bacteria of the Staphylococcus spp. group most often were carriers of the ErmB gene, which is responsible for resistance to macrolides of the 1st generation. E. coli had a high percentage of resistance to inhibitorprotected penicillin and cephalosporin of the 3rd and 4th generations. Acquired resistance of E. coli and bacteria of the Staphylococcus spp. group (S. epidermidis, S. saprophyticum, S. haemolyticus) is associated with the frequent use of antimicrobials in the agricultural enterprises, such as penicillin, 3rd and 4th generation cephalosporin and 1st generation macrolides. Also the poly-resistant E. coli isolates (2.2%) resistant to several groups of antimicrobial drugs were detected in the cow milk samples. Keywords— resistance, gene, DNA, antimicrobial drugs, bacterial pathogens, genetic determinants, milk.

Genetic markers of antibiotic resistance of pathogenic bacteria in the milk of cows and goats