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The main aim of the study was to isolate and identify any organism that can degrade 2,2dichloropropionic acids (2,2DCP) from Antarctica soil and water environment. Antarctica is well known of its extreme cold temperature. In this study we successfully isolated at least six colonies (AA1, AA2, AB1, AB2, AC1 and AC2) from Antarctic environment of the soil sample grown on Antarctic Bacterial Medium. Of the six isolates, only isolate AA2 was could grow on a minimal media containing 2,2-dichloropropionic acids as a sole source of carbon. Analysis of the 16S rRNA gene sequence and biochemical tests, identified strain AA2 belongs to Bacillus sp.. The bacterium showed slow growth below 5C, with cells doubling time of 32 hours. The chloride ion released was 0.657 mmolCl/L on 20mM of 2, 2-DCP. In conclusion current study is important to explore biotechnological applications of cold adapted organisms and its special properties for fundamental applications. The new isolate can be used to improve the present bioremediation methods at low temperature. This is the first reported case about microbial growth on 2,2DCP (DALAPON) using a bacteria isolated from Antarctica.

Putative non-stereo specific dehalogenase from Antarctic of Psychotropic Bacillus sp IH01