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To enable hazard-free treatment of swine manure, a bacterium capable of using oxytetracycline as the sole source of carbon (Staphylococcus sp. TJ-1) was isolated. The organism was able to degrade 80.7% of oxytetracycline in 5.5 d when cultured at pH 7.0, 30°C and 100 mg/L of oxytetracycline. For its application in engineering, the key conditions for composting were optimized using the custom design. When setting the conditions as 70% of the initial percentage of moisture, an initial C/N of 30, inoculation with 1% TJ-1 and the stack-turning every 1.5 d, the degradation of oxytetracycline reached 98.2%, indicating that the organism could be used for agriculture application to reduce the oxytetracycline pollution. Keywords-oxytetracycline degradation; compost of swine manure; bioaugmentation; Staphylococcus sp. TJ-1

Isolation, Identification, and Bioaugmentation of an Oxytetracycline-Degrading Bacterium Staphylococcus sp. TJ-1 in Composting of Swine Manure