English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Tetracycline has been widely used as a periodontal support treatment since it has broad-spectrum bone tissue penetration and it can inhibit native collagen. Chitosan hydrogel-based tetracycline is known to have strong antibacterial effects, but the cytotoxic effects on fibroblast have yet to be studied. The objective of this study is to obtain the in vitro cytotoxicity effect of chitosan hydrogel-based tetracycline on fibroblasts. Chitosan hydrogel-based tetracycline (0.5%, 0.7% and 1%) and chitosan hydrogel without tetracycline is made by dissolving chitosan powder in citric acid. Tetracycline powder is then added to the solution. 3T3 fibroblast cells were cultured in a well microplate containing RPMI-1640 media inside an incubator. The viability assay is conducted using the MTT-assay method and repeated 5 times. The colour degradation is read with a microplate reader. The mean viability of fibroblasts applied with chitosan hydrogel-based tetracycline (0.5%, 0.7% and 1%) and chitosan hydrogel is 86.5%, 85.36%, 80.99% and 90.85%, respectively. The highest mean cell viability in the group of fibroblasts applied with chitosan hydrogel-based tetracycline is in the 0.5% group. This value is not significantly different than the value in the 0.7% group, but it is significantly different than the value in the 1% group. Chitosan hydrogel-based tetracycline shows a low cytotoxic effect on 3T3 fibroblast cells.

In Vitro Chitosan Hydrogel Based Tetracycline Cytotoxicity Test on Fibroblast Viability