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Avian Influenza virus (AIV) have caused significant economic losses in poultry sector worldwide. Results of genetic mapping showed that circulating AIV in Indonesia were belong to clade 2.1.1, 2.1.2, 2.1.3 and 2.3.2, however the AIV subtype were merely H5N1.Our study aim to identify the hemagglutinin protein of AIV isolated from native laying duck in Sidenreng Rappang, South Sulawesi, Indonesia using HI assay. Three cloacal swab samples:Sidrap3 (S3), Sidrap5 (S5), and Sidrap10 (S10) were isolated from native laying duck at three duck farms at Sidenreng Rappang during AI outbreaks in 2016. These samples were propagated on spesific antibody negative (SAN) embryonated chicken eggs, followed by subtype identification using hemagglutination inhibition (HI) test using specific antibody of AIV subtype H5N1, AIV subtype H5N2, AIV subtype H5N3 and ND Lasota virus. Our study showed that H5N1 specific antibody has titer of 2 4 against virus isolates Sidrap3 and Sidrap10, titer of 2 6 against virus isolates Sidrap5. H5N2 specific antibody has titer 2 4 against virus isolates Sidrap3 and Sidrap5, titers of 2 6 against virus isolates Sidrap10. H5N3 specific antibody has titers of 2 6 against virus isolates Sidrap3, Sidrap5 and Sidrap10. Whilst, specific antibody of ND Lasota did not react with all of virus isolates. Our results showed that all AIV isolates gives highest reaction with specific antibody of AIV H5N3. According to the result, it is likely that AIV H5N3 might circulating among laying duck in Sidenreng Rappang.

Subtype Identification Of Avian Influenza Virus Isolated from Laying Duck In Sidenreng Rappang, South Sulawesi With Hemagglutination Inhibition Assay