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Fish scale is a newly developed alternative resource for extracting collagen,which is one of the important biological materials. To extract collagen from fish scale has a great significance to prevent pollution and receive good economic benefits. In this paper, based on the experimental results of single factor test, the response surface method was used to further optimize the technical parameters for the decalcification of bighead fish scale and establish the process model and kinetic model. The results showed that the maximum calcium removal rate reached 93.40% under the optimal conditions of liquid/solid ratio 23.50, citric acid concentration 11.0%, decalcification time 66.3 min and magnetic steering speed 989r/min and the maximum decalcification velocity reached 241.86mg/(L· min) under the optimal conditions of liquid/solid ratio 10.10, citric acid concentration 12.60%, decalcification time 31.6 min and magnetic steering speed 865r/min. The two models in the form of multiple regression were proved to basically agree with the test values and can be used to predict the calcium removal rate and decalcification velocity under different operation parameters and the decalcification velocity corresponding to the calcium removal rate under same parameters, to adjust the operation parameters for obtaining the expected calcium removal rate and decalcification velocity, and to control the production capacity of fish scale treatment and the collagen quality prepared by following collagen extraction from the decalcified fish scale.

Optimization and Models of Decalcification from Bighead Carp Scale with Citric Acid by Response Surface Method