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In industrial processes large amount of cost and pollution produced are derived from the process of removing lignin (delignification) to make high-quality paper through the chemical pulping process. Rynchophorus ferrugineus are wood-feeding insect that have the capacity to degrade wide range of lignocellulosic materials. Within this insect, the gut microbiomes play a major role in degrading lignocellulosic materials efficiently. The purpose of this research is to identify potential bacterial gut that could degrade lignin compound. R. ferrugineus larvae were dissected and the gut microbiomes were isolated. The bacteria were cultured in minimal salt media (MSM) with 0.5% lignin as sole carbon source. With this selective media, bacteria with lignin ability were cultured. Fifteen colonies were selected for species identification via 16S rRNA sequencing using universal primers 518F/800R which targets on 16S gene. The capacities of individual species to produce ligninase were observed via enzyme activity assay. The results revealed four different types of species with lignin degrading ability that reside within the gut which are Enterobacter sp., Serratia sp. and Pseudomonas sp.. The ability of these bacteria in degrading lignin compound holds huge potentials which are useful for various applications such as bio-pulping, bioremediation and biofuel.

Potential lignin degraders isolated from the gut of rhynchophorus ferrugineus