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In this research, a novel and facile method for preparing organic-inorganic hybrid microspheres was developed by a synergy of membrane emulsification and biomimetic mineralization. The gelatin solution (water phase) was extruded through a Shirasu Porous Glass (SPG) membrane, and mixed with the liquid paraffin (oil phase) to form a monodispersed W/O emulsion. After cooling and cross-linking, the organic gelatin microspheres were obtained, and then immersed in to Na2SiO3 solution, which acted as silicon precursor. Under the catalysis of gelatin, silica coating was formed around the gelatin microspheres, resulting in the final gelatin/silica hybrid microspheres. The as-prepared hybrid microspheres were subsequently utilized for encapsulation of β-Glucuronidase (GUS). The results indicated that the recycling and storage stability of the immobilized enzyme are all significantly improved.

Facile construction of novel organic-inorganic hybrid microspheres for enzyme immobilization