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Currently, the greenhouse effect is becoming increasingly serious, and biological carbon-fixation, especially microbial biological carbon-fixation, can play a unique role in curbing the greenhouse effect.CO2 and N2 are the major greenhouse gases. In this study, a facultative CO2 and N2 fixing bacterium named BHJ (which can fix CO2 and N2 simultaneously) was identified using molecular biology, and its growth conditions were determined. Total DNA from BHJ was extracted and then the 16S rDNA was amplified using polymerase chain reaction. The 16S rDNA sequence was identified and analyzed by alignment with sequences in the GenBank database. Single variables in medium without carbon and nitrogen (KH2PO4 0.2 g, CaCl2 formula: 0.2 g, NaCl 0.2 g, MgSO4 0.3 g, VB 0.0625 g, trace element solution 0.5 mL, distilled water: 1000 mL) was performed. Spectrophotometry was used to measure the optical density changes to determine the optimum temperature, CO2 and N2 ratio, and pH for the growth of BHJ. The 16S rDNA sequence of BHJ showed more than 99% homology with that of Burkholderia cepacia. For the growth of BHJ, the optimum temperature was 34°C, the best concentration ratio of CO2 and N2 was 1:99, and the optimum pH was 5.

Molecular Biological Identification and Determination of Growth Conditions of Facultative CO2 and N2 Fixing Bacterium BHJ