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Polyacrylamide (PAA) is a reticular macromolecular compound, and the C=N bonds produced by the reaction with glutaraldehyde reveal intensive near-infrared fluorescence (NIRF). This paper studies the effect factors on the fluorescence intensity of PAA microspheres (beads), including the particle size of PAA beads, APMA concentrations, and different concentrations of glycine treatment by infrared imaging system and fluorescence microscope. The results demonstrate that PAA gel and PAA beads display autofluorescence at the emission wavelength of 700 nm and 800 nm,respectively. The smaller the particle size of beads is, the better the fluorescence intensity is in a certain range. The beads have stronger NIRF when 11 mg/mL APMA is in monomer liquid compared with the beads containing 7 mg/mL or 9 mg/mL APMA in monomer liquid. Glycine treatment enhances the fluorescence intensity of the beads, which is the most obvious at 0.2 M glycine in solution.

Near-infrared Fluorescence Imaging and Optimization of Polyacrylamide