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This paper presents the intrinsic fluorescence characteristics of vitamin B1 and vitamin B2 with 3D fluorescence Spectrophotometer. Three strong fluorescence areas of vitamin B2 locate at λex/λem=270/525nm, 370/525nm and 450/525nm and one fluorescence areas of vitamin B1 locates at λex/λem=370/460nm were found. The influence of pH of solution are also discussed, and with the PARAFAC algorithm, 9 vitamin B2 and vitamin B1 mixed solutions are successfully decomposed, and the emission profiles, excitation profiles, central wavelengths and the concentration of the two components were retrieved precisely through about 10 iteration times.

Study on Fluorescence Spectra of Thiamine and Riboflavin