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A new method for the determination of Gemifloxacin (GMFX) indirectly was developed by using energy transfer of Acridine Orange (AO) Rhodamine B (RB) fluorescence resonance. It was found that in the pH=7.00 Britton-Robinson (B-R) buffer solution and sodium dodecyl sulfate (SDS) medium, effective energy transfer could occur between AO and RB,the fluorescence of RB was enhanced. The fluorescence intensity of RB quenched with the addition of the GMFX. Using this method to determine the GMFX tablet, the results were consistent with high performance liquid chromatography (HPLC) method, the sample recovery was 98.58~102.16%, the relative standard deviation (RSD) was 0.68 ~2.2%, the detection limit of this method was 0.025mg • L. Experiments showed that this method was simple, rapid, accurate, and sensitive. Keywords-Gemifloxacin; fluorescence resonance energy transfer; fluorescence quenching; Acridine Orange; Rhodamine B

The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin