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The Drosophila hsp90 gene can response to responses to temperature stress and all kind of infection. However, the mechanism of how the hsp90 gene is mediated is still unclear. In the present study, we proved that hsp90 gene abundantly expressed during molting and metamorphosis stage. 20-hydroxyecdysone (20E) treatment can induce hsp90 gene expression, the knockdown of USP1 and EcR-A led to a reduced transcript level of hsp90 gene in Drosophila S2 cells. Those results certified that the Drosophila hsp90 gene involved in the ecdysone signaling transduction pathway and act downstream of the ecdysone receptor.

Drosophila Hsp90 Gene Can be Upregulated by Ecdysone