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Sugar esters are nonionic surfactants. They are widely used in the food, cosmetic, detergent and pharmaceutical industry because of its high emulsifying capacity, non-toxicity, wide range of HLB value (1-16) and biodegradability. In addition, sugar esters have the effect of lubricating, detergency, foaming, anti-ageing, anticrystallizing and anti-bacteria etc. Sugar esters are recommended food additives by FAO and WHO. Enzymatic synthesis has many advantages over conventional chemical synthesis of sugar esters. It is very important to carry out the study of enzymatic synthesis of sugar ester in non-aqueous system. By the single factor experiments, the effects of reaction system, the content of sugar, the reaction temperature, the addition amount of lipase, the addition amount of molecular sieve and the reaction time on sugar ester transformation were investigated. On the basis of single factor experiments, the perpendicular experiments were designed to optimize the condition. The optimal conditions were built when the reaction was carried out in the tert-butyl alcohol with 0.6 mol/L of lauric acid, 0.08 mol/L of sucrose, 30 g/L of lipase and 50 g/L molecular sieves, at 50°C for 65 hours. Keywordssugar ester; lipase; enzymatic non-aqueous system

The Lipase-catalyzed Synthesis of Sugar Ester in Non- aqueous Medium