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A 30-day incubation experiment was conducted to investigate the effects of a long-effect additive (NAM), dicyandiamide (DCD), and glucose additions on N transformation in soil. The results showed that both NAM and DCD enhanced N transformation into soil microbial biomass N and fixed NH4+. Glucose addition enhanced transformation of N into soil microbial biomass N, consequently decreased soil mineral N and fixed NH4+ At the end of the incubation, NAM favored to increase soil mineral N, indicating the higher availability of N. Overall, our research identified that N can be stabilized in soil by the processes of microbial immobilization and mineral fixation, which were enhanced with the addition of NAM and DCD, reducing N losses and remaining N availability, especially for NAM. Although organic C addition increased microbial immobilization of N, the availability of N was significantly lowered. Therefore, the rate and time of organic C application should be regulated to optimize N management practices. Keywords-NAM; DCD; urea; soil microbial biomass N; fixed NH4+; glucose

Effects of the Long-effect Additive (NAM), Dicyandiamide (DCD), and Organic C Additions on N Transformation in Soil