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The co-culture of microorganisms involves at least two microorganisms or more in an environment of confined conditions, which is induced by microbe communities that are ubiquitous in nature. Therefore the attentions of people are increasingly attracted to utilize the co-culture approach in many fields. The growth of microorganisms should be detected starting from the isolation medium and each of them should be recorded respectively which are of significant importance in the process of co-culture. In this paper, L.paracasei HD1.7 and B.subtilis were applied to establish the isolation method. The growth of each of them was determined in MRS and BP medium. The results demonstrated that L.paracasei HD1.7 was not able to grow on BP medium cultivated at 37 °C for 36h, while B.subtilis could not grow on MRS medium incubated at 37 °C for 30h. Keywords-co-culture, L.paracasei HD1.7and B.subtilis, isolation method

Establishment of Isolation Method by Co-Culturing of L.paracasei HD1.7and B.subtilis in Two Media