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The Balb/c 3T3 cell transformation assay (CTA) is presently undergoing extensive pre-validation testing but its potential for routine chemical screening remains to be fully exploited due it being more costly and more time consuming than other CTAs. To downscale the assay 24-well plates were used to score foci and then compared to the standard T25 culture bottles. Furthermore, it was also attempted an adaptation to a perfusion cell culture model in order to achieve a closer equivalent to the in vivo carcinogenic processes. The miniaturized approach was successfully correlated to the standard technique. A reproduction in smaller culture plates can provide an alternative for a faster and more practicable handling by smaller laboratories. The assay failed to produce positive results in the fluidic model and further testing is necessary to determine the role medium flow in the in vitro carcinogenesis process.

Analysis on the Performance of the Balb/c 3T3 Two-stage Assay in a Miniature Culture Area and in a Perfusion Culture Model