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Objective: To study the changes of natural killer T cells (NKT cells)in peripheral blood and the ability of NKT cells to proliferate in response. Methods: 80 bronchial asthma children and 40 healthy children were included. NKT cells and T cells were analyzed by flow cytometry instrument. The levels of IL-4, IFN-γ, IL-10 were tested in different period of NKT cell culture. Results: There were 46 cases were acute, 34 cases were remission. Between the three groups, CD4+, CD8+, CD4+/CD8+ and NKT cells percentage comparison difference were statistically significant (P 0.05). With the extension of incubation time, IL – 4 levels in three groups of children were significantly increased, and acute attack group were higher than that in remission group and normal group. The difference was statistically significant (P < 0.05). Serums IL 10 in cultivating 48 h raised highest, cultivate 72h declined, and acute attack group were higher than that in remission group and normal group. The difference was statistically significant (P < 0.05). Conclusion: The pathogenesis of asthma may be related to the ratio and dysfunction of NKT cells.

The Study on Changes of NKT Cells Levels in Peripheral Blood of Children with Asthma