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To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Pulsatilla chinesis (Bge.) Regel., we extracted the genome DNA from the leaves of Pulsatilla.dahurica (Fisch.) Spr., collected in Jilin Province, amplified the ITS and trnL-F region using ITS universal and trnL-F primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here. Keywords-Pulsatilla.dahurica (Fisch.) Spr.; ITS; trnL-F; Sequencing: Authentication.

ITS and Trnl-F Sequences Analysis of Pulsatilla.Dahurica (Fisch.) Spr.