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A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of cry9Ea10 was cloned and sequenced. The engineering strain of Bt carrying cry9Ea10gene wasconstructed successfully. SDS-PAGE analysis confirmed that the expression of cry9Ea10 as ~130kDa protein. And Cry9Ea10 protein produced by Bt engineeringstrainexhibited a high toxicity to H.cuneal arvae.

Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae