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Purpose: To study the effect and function mechanism of Tonifying Kidney and Spleen, Detoxification of Throat method on IgA nephropathy. Methods: the 72 Wistar rats were randomly divided into blank group, model group, Lotensin group, Tonifying kidney and spleen, detoxification of throat high, medium and low dose group (which is called traditional Chinese medicine high, medium and low dose group for short). The IgA nephropathy rat model were established by the method of feeding with bovine serum albumin (BSA) and injecting tail vein with lipopolysaccharide (LPS).After Eight weeks administration, each phase’s urine red blood cell count, 24h UTP, TNF-α and IL-6 content were observed. Results: compared with model group, every groups’ UTP decreased and TCM medium dose group decreased extraordinary (P<0.05);compared with model group, urine red blood cell count decreased significantly of every TCM group(P<0.05), especially TCM medium dose group improved significantly (P<0.01), but Lotensin group urine red blood cell count no drop (P>0.05); compared with blank group, TNF-α,IL-6 of model group’s were significantly elevated (P<0.05);compared with model group, TNF-α,IL-6 of treatment groups decreased significantly(P<0.05),compared between TCM group, TCM medium dose group's data is the most significant(P<0.01).Conclusion: Tonifying kidney and spleen, detoxification of throat method can reduce hematuria and proteinuria of IgA nephropathy model rats, and reduce the content of IL-6 and TNF-α in serum which is better than Lotensin. Introduction IgA nephropathy (IgAN) is a relatively common glomerular disease with high incidence in our country, accounting for about 30% ~ 40% of chronic nephritis. It is one of the leading cause of end-stage renal disease [1-2]. The pathological feature is that glomerular mesangial area appears diffuse IgA deposits with mesangial proliferation, its mainly clinical manifestation is hematuria and proteinuria. Tonifying kidney and spleen, detoxification of throat method was come up by national physician master Jixue Ren.It can effectively reduce the IgAN patient's hematuria and proteinuria, delay the deterioration of renal function. In order to further study the function mechanism of this method in the treatment of IgAN, the paper makes a research, the reported as follows. International Conference on Biomedical and Biological Engineering (BBE 2016) © 2016. The authors Published by Atlantis Press 221 Materials and Methods Animals Choose 72 health and clean male Wistar rats in 6 weeks old, the weight is 160 ~ 200g, provided by animal laboratory, the Norman Bethune medical college of jilin university, experimental animals qualified number: SCXK (liao) 2013-000. Instruments Reagents and Drugs TXM-500C fluorescence microscope, provided by Shanghai Tiansheng instrument co.; Automatic enzyme standard meter 318C, provided by Shanghai Zuofei laboratory equipment co.; Organized crusher BIO 950, provided by Shanghai Bannuo biological technology co.; TNF-αand IL-6 ELISA detection kits, provided by the Shanghai Xinyu biological technology co.; Bovine serum albumin and lipopolysaccharide, provided by America Sigma company; Fluorescein label rabbit sustain rat IgA antibodies, provided by Beijing Boorson biological technology co.; Lotensin provided by Beijing Novartis pharma co.(its national standard number: 1050423H0); Drugs towards Tonifying Kidney and Spleen, Detoxification of Throat method all supervised by the Affiliated Hospital to Changchun University of Chinese Medicine. The Method of Experiment Establish the Model. Firstly, feed the rats a week after purchase for letting them adapt the environment; secondly, randomly divided them into blank group, model group, Lotensin group, Tonifying kidney and spleen, detoxification of throat method high, medium and low dose group; thirdly, prepare ainimal model by referring to huiyu Liu[3] suggested method, and select 2 rats from each group in the ninth week; fourthly, check whether successfully establish the model by choosing nephridial tissue under microscope. Grouping and Drug Delivery. After the successfully establish the model, the control group is given Lotensin 10mg/(kg·d) by gavage administration; the treatment group is given the dose of traditional Chinese medicine high, medium and low 32, 16 and 8g/(kg·d) by gavage administration; the blank group and model group is only given saline lavage 4ml a day to the stomach. All of these continually to 8 weeks. Observation. Observe the general condition of rats, including body quality, the hair color, the urine color and urine output, respectively at the weekend of 0, 4, 6, 8, 10, 12, 14 collect the 24h urine by metabolic cages. Then observe its color, record urine quantity, the frequency and degree of macroscopic haematuria, take part of urine to detect by UTP in 24h, check the count of urine sediment and urine red blood cell, and detect the content of TNF-αand IL-6 in serum by ELISA method( operate under the guide of kit introduction)

Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to TNF-a#945;, IL-6 of IgA Nephropathy Model Rat