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Rosa chinensis is being widely investigated because of its extremely high ornamental value and commercial value. Scientists have entered the molecules for its researching level. Extraction of high quality RNA is a primary and key step towards undertaking molecular biology experiments, which is often affected by contaminants, such as pigments, polyphenols, proteins, plant secondary metabolites, and genomic DNA from plant tissues. Here the authors build a new protocol suitable for isolating high-quality RNA from the petals of Rosa chinensis. Based on a CTAB method, the quality of RNA is very pure with little contaminants and makes a figure in gel electrophoresis. After RT-PCR, the 860 bp sequence of chalcone synthase (CHS) gene was obtained to identify the quality of total RNA from Rosa chinensis. In these experiments, The RNA isolated by the improved CTAB method is quite excellent.

Isolation of Good-Quality RNA from Rosa chinensis, Rich in Secondary Metabolites