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To better understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene HTH was cloned from blackberry. The open reading frame of this gene was 1785 bp, encoding 594 amino acid residues with a molecular weight of 64.6 kD and a theoretical isoelectric point of 9.39. Its amino acid sequence has the highest identity with strawberry. The results of real-time quantitative PCR showed that the expression level of HTH gene decrease rapidly with the maturity of the fruit. The expression level of HTH decreased with the increase of red or blue light intensity. The lowest expression of HTH gene was under pure blue light treatment.

Expression and Sequence Analysis of HTH in Blackberry (Rubus Spp.)