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Vegetative breeding that is mostly done now is through tissue culture techniques. The objective of this research was to obtain a description and examine the influence of the growth regulators 6Furfuryl amino purine (KIN) and naphthalene acetic acid (NAA) on the formation of in vitro shoots in this cultivar. For this reason, testing is needed to determine the characteristics of the in vitro shoots produced which can be useful as a tool for the formation of synthetic seeds. The ability of cotyledon explants to form the highest shoots (70%) was in the treatment containing 2 mg/l KIN + 0,5 mg/l NAA but this treatment did not show a significant difference with 2-3 mg/l KIN and 2 mg/l KIN + 0,5 mg/l NAA. The percentage of shoot formation correlates closely and positively with the number of culture bottles that form shoots (r = 0.72). Based on the description data and the result of the selection of the number of shoots selected two treatment of shoot induction i.e. treatment of 2 mg/l KIN + 0.5 mg/l NAA which has stem shape of round and straight, and treatment of 1 mg/l KIN + 0.5 mg/l NAA which also has the same stem shape. Shoots of selected induction results can be used for the formation of synthetic seeds. Keywords—Broccoli, 6-Furfuryl Amino Purine, naphthalene acetic acid, in vitro, description, characteristics, shoot formation, cotyledon, synthetic seeds

Characterization of in Vitro Shoots Brassica oleracea Results of Cotyledon Explants Induction Using KIN and NAA