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The SiO2 microspheres with different diameters were prepared by a seeding technique through adjusting the mass fraction ratio of TEOS and silica sol in the reaction solution. The mesoporous SiO2/Fe3O4 microspheres with size-tunable hollow cavity were prepared by using the SiO2 microspheres of different diameters as templet. The magnetic mesoporous SiO2/Fe3O4 hollow microspheres were used as the carriers to immobilize papain, and the effect of hollow cavity on papain immobilization was researched in detail. The results show that the amount of papain immobilization increased with the size of hollow cavity increasing. The amount of papain immobilization washigh(296 mg·g -1 ), when the hollow cavity average size was about 330nm. It was indicated that the hollow cavity is a natural warehouse storing papain molecules effectively. When the temperature was below 60℃ and the pH was from 2 to 8, the residual activity of immobilized papain still remained more than 60%, much higher than that of free papain.

The Preparation and Papain Immobilization of Novel Mesoporous SiO2/Fe3O4 Microspheres with Size-tunable Hollow Cavity