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Mansonia is one of the genera of mosquitoes that play a role in the transmission of filariasis in North Hulu Sungai Utara undertook mass treatment of entire endemic communities from 2006 to 2015 to eliminate the transmission of the disease. However, post validation surveillance activities are required to ensure the gains achieved are sustained. Entomological survey was carried out to assess the mosquito vectors of the disease and determined the presence of infection in these vectors, testing the hypothesis that transmission has already been interrupted in North Hulu Sungai District. The study was conducted in Pihaung Village which is an endemic filariasis village, which adult filarial mosquito vectors were collected by using double net-trapped human bait method with modification. Abundance, species dominance were analyzed and PCR-based to determine DNA filarial of suspected vector. Total of 942 mosquitoes from five genera Anopheles, Aedes, Armigeres, Culex and Mansonia were captured at both sites. Of which, Mansonia has the highest relative abundance 29.96%. In this study periode resulted Mansonia dives Man Hour Density and Man Biting Rate were 4.7 and 10.5 respectively. PCR examination were detected Brugia malayi DNA on Mansonia as suspected vector in Pihaung Village. This study conclude the existing of Brugia malayi DNA in this area highlighting potential risk for the re-emergence of LF transmission. This study recommends routine vector control and strengthen surveillance is required to continuously monitor the filarial suspected vector density.

Detecting Brugia Malayi in Lymphatic Filariasis Mosquito Vector in North Hulu Sungai District, South Kalimantan, Indonesia