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Yeasts growth media substitution in producing mannoprotein has become important recently. Bean sprouts were developed as nitrogen sources alternative for yeasts growth media. The research aims to determine the role of bean sprout as alternative growth media in producing mannoprotein by Candida apicola, Trichosporon beigelli and Saccharomyces cerevisiae. The extract of 3% bean sprout broth were inoculated by three-loop of yeasts i.e. C.apicola, T.beigelli and S.cerevisiae then incubated at 30 0 C for 75h. Yeasts growth on bean sprout broth was identified every 5h for optical density (OD), pH and mannoprotein biomass. Results showed that all of the yeasts shown good growth at bean sprout media, with C.apicola shown the highest optical density of 2.0670A, pH of 4.3 and mannoprotein biomass of 0.278 mg/ml. Trichosporon beigelli shown optical density of 1.5383A, pH of 4.6, and mannoprotein biomass of 0.0156 mg/ml. Meanwhile, Saccharomyces cerevisiae resulted in optical density of 1.3949A, pH of 4.4, and mannoprotein biomass of 0.0102 mg/ml.

Mannoprotein Production by Candida apicola, Trichosporon beigelli, and Saccharomyces cerevisiae in Bean Sprouts Media