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The resistance of Anopheles spp to insecticides is the most important and dangerous problem that affects malaria control programs. One of the methods used to detect insecticide resistance is the bottle bioassay test method from Centers for Disease Control (CDC) which can be applied as part of a broader insecticide resistance monitoring program. This study aimed to determine the resistance of Anopheles spp. against the insecticide cypermetrin at the location of malaria cases in 2018 in Sabang City. The locations for resistance tests were carried out in Balohan, Batee Shok and Iboih areas. The resistance test of Anopheles spp. against cypermethrin insecticide was carried out using the CDC bottle bioassay method with 100 mosquitoes per location. The resistance data were analyzed using probit regression. The results of resistance testing against the insecticide cypermethrin showed that the mortality rate of Anopheles spp in three different locations, namely Balohan with a mortality rate of 3%, Iboih 98% and Batee Shok 99%. The LT50 values of the Balohan were 101.47 minutes, Batee Shok 25.38 minutes, and Iboih 13.50 minutes, with resistance ratio values of 16.67, 1.82 and 1.02, respectively. It can be concluded that Anopheles spp. in Balohan is resistant to the insecticide cypermethrin, while Anopheles spp. found in Batee Shok and Iboih areas are classified as susceptible to the insecticide cypermethrin.

Resistance Test for Anopheles spp. to Cypermethrin Insecticide Using Bottle Bioassay Test Method