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The change of paradigm in forest product management brought out the Forestry Minister's Regulation Number P.35/Menhut-II/2007, concerned Non-Timber Forest Products (NTFPs). Some genus Cinnamomum included in the list of NTFPs, and Cinnamomum camphora (camphor) is one species. The recorded data mentioned that this plant was found abundant in Kalimantan and used as a complement in traditional ceremonies, especially for the Dayak Tunjung community, in Pepas Asa village. This research aimed to explore further the potential of C. camphora essential oil grown in East Kalimantan as antibacterial. Essential oil fractions obtained from the leave of C. camphora (CP), which grew in Pepas Asa village, were fractionated by water and steam distillation based on their distillation models (CP5, CP7, and CP9) and separation times (0-1 h, 1-2 h, and 2-3 h). A hand refractometer physically analyzed the oils to determine their refractive index value. The essential oils were also examined for their antibacterial activity at a concentration of 1%, 10%, and 100% (pure oil fraction) by agar diffusion assay against Streptococcus mutans. The results showed the highest yield of oil fractions for each model were 1.62% (CP51), 2.35% (CP71), and 1.23% (CP91. The result of antibacterial activity showed that all fractions from three models potential as antibacterial agents in the concentration of 100% (pure oil), especially the fraction of second-time distillation, could inhibit the growth of S. mutans higher than tested positive control.

Effectiveness of Distillation Models on Bioactivity from Essential Oil Fraction of Cinnamommum camphora (L.) J. Presl.