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Corymbia maculata  stands out for its resistance to biotic and abiotic stress and for its high-density wood, which is ideal for sawmills, sleepers, posts, firewood and charcoal.  In vitro  culture techniques can be used for large-scale clonal microplant production, given the difficulty in adventitious rooting via cutting or mini-cutting techniques. The aim of the work was to evaluate the  in vitro  multiplication, elongation and adventitious rooting stages of  Corymbia maculata  based on chemical sterilisation of the culture medium, plant growth regulators, gaseous exchange, activated charcoal and growth room lighting. Supplementation of active chlorine in culture medium reduced fungal and bacterial contamination. The best result for the  in vitro  multiplication stage was observed in the absence of active chlorine and 0.5 mg l −1  benzylaminopurine (BAP).  In vitro  shoot elongation in culture medium supplemented with 0.5 mg l −1  α-naphthaleneacetic acid (NAA), 0.001% or 0.003% active chlorine, and vessels sealed with a rigid polypropylene lid with one or three membranes was the most suitable. Glass flasks with porous membrane lids allowed gaseous exchange and favoured elongation and adventitious rooting. Microshoots treated with indole-3-butyric acid (IBA) for 48 h (pulse effect) in the absence of light and activated charcoal in the culture medium is recommended for  in vitro  adventitious rooting of  Corymbia maculata .

southern forests: a journal of forest science

Clonal microplant production of  Corymbia maculata : effect of chemical sterilisation, plant growth regulator, gas exchange, activated charcoal and lighting