English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Metabarcoding to determine marine zooplankton species composition is a fast-developing method, yet to be fully standardised. DNA barcode reference libraries that link species to barcode sequences remain incomplete, taxonomically imprecise, and biased towards well-studied regions. We used metabarcoding to determine the decapod crustacean species present in the marine zooplankton off eastern South Africa, a region for which reference libraries are comparatively poor. Zooplankton communities were sampled with tow nets at stations on the shelf (20- and 50-m isobaths) and at the shelf edge (100- and 200-m isobaths), and the samples were processed using high-throughput sequencing technology. The cytochrome c oxidase subunit I (COI) gene region was used for taxonomic assignment of amplicon sequence variants to species level at 95% and 99% similarity to barcode records. Detected species were cross-referenced against occurrence records from the region. Eighty- one decapod species were detected at 95% sequence similarity, but this declined to 60 species at the stricter 99% threshold. False-positive identifications were reduced by 60%. True crabs (Brachyura) were proportionally over-represented; the ratios of prawns (Dendrobranchiata), lobsters (Achelata) and burrowing shrimp (Axiidea) were consistent with occurrence records; and true shrimps (Caridea) and hermit crabs (Anomura) were under-represented. Metabarcoding identified 19 tropical western Indian Ocean (WIO) species in the samples from eastern Africa, confirming a southwards dispersal of drifting phases through the Mozambique Channel. Congruence of WIO species with the Agulhas Bioregion (shelf-edge detections) and Delagoa Biozone (shallow detections) was consistent with the dispersal of tropical species in warmer water masses. Metabarcoding of marine zooplankton communities to obtain species-level information advances high-resolution ecological research in pelagic ecosystems.

Metabarcoding of zooplankton confirms southwards dispersal of decapod crustacean species in the western Indian Ocean