English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The study was conducted to barcode five freshwater fish species obtained from Nile River tributaries (the Atbara River and the Blue Nile), in March 2019, using analysis of partial CO1 gene sequences. Fish samples were identified morphologically as Nile perch  Lates niloticus , Nile labeo  Labeo vulgaris , North African catfish  Clarias gariepinus , Cornish jack  Mormyrops anguilloides  and bebe mormyrid  Hyperopisus bebe . Following DNA extraction, universal primers were used for amplification of the CO1 barcode region. The PCR products were sequenced and used for molecular identification. Each sequence was matched to corresponding GenBank sequences using the BLAST technique. Sequences of all specimens were aligned along with the GenBank published sequences and polymorphic sites were identified. The analysed sequences were uploaded to GenBank under the accession numbers OL804282– OL804286. The analysed partial CO1 barcode gene showed a readable length of ∼548–669 base pairs. Sequence comparison of CO1 gene sequences with sequences published in GenBank revealed 99.27–100% identities at the species level. Therefore, the CO1 barcodes accurately identified all the studied freshwater fishes. This is the first molecular DNA barcode characterisation of freshwater fish species found in Sudan. The fish CO1 gene sequences revealed in this study will serve as barcode references for future characterisations of Nile River fishes.

DNA barcoding of five economically important freshwater fish species from the Nile River, Sudan