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This study evaluated the interactive effects of low rates (0, 25, 50 and 75 kg N ha −1 ) of nitrogen (N) fertilizer application and stage of maturity on total N, N fractions and  in vitro  ruminal N degradability in  Brachiaria  hybrid,  Megathyrsus maximus  and  Paspalum atratum . The grasses were harvested at 4, 8 and 12 weeks regrowth, except for  P. atratum  that was harvested at 6 and 12 weeks. There was a significant N fertilizer rate × stage of maturity interaction ( p  &lt; 0.001) on total N, buffer soluble N (BSN), buffer insoluble N (BISN), acid detergent insoluble N (ADIN) and  in vitro  ruminal N degradability (ND) in all grass species. Total N, BISN and  in vitro  ruminal N degradability increased linearly with N fertilizer rate up to 50 kg ha −1  in  Brachiaria  hybrid and  M. maximus  and were highest in grasses harvested at 4 weeks maturity. Nitrogen fertilization rate was positively correlated with total N, BISN ( r  = 0.70 to 0.97) and ND ( r  = 0.60 to 0.98) in  Brachiaria  hybrid and  M. maximus  forage. We concluded that N fertilisation rate of 50 kg ha −1  per application interval is adequate to improve N, N fractions and N degradability of tropical grass forage.

Low nitrogen fertiliser rates and stage of maturity influence nitrogen fractionation and in vitro ruminal nitrogen degradability in tropical grasses