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Background and Aims:Activin was originally found as a stimulating factor of follicle stimulating hormone in pituitary cell cultures. To establish a simple culture system for studying the effects of activin on prolactin productionwe examined the effects of activin in rat somatolactotroph derived GH3 cells cultured in a serum free condition. Methods:GH3 cells were cultured in Hams F10 medium contain‑ ing 2 mM L-glutamine and 0.1% (w/vBSA with activinfollistatin (an activin binding proteinor a vehicle(control. Results:The cell viability was between 80 to 90% throughout the 96 hour incuba‑ tion period in the three groups. Activin stimulated prolactin production in a time dependent (24-96 hoursand dose dependent(75-300 ng/mlmanner. A co-treatment with graded doses of follistatin and 100 ng/ml of activin suppressed the increase of prolactin production brought about by activin in a dose dependent mannerand the production decreased to the control level after the addition of 300 ng/ml of follistatin. Conclusions:The present study demonstrated the stimulatory effect of activin on prolactin production and the antagonism between activin and follistatin in GH3 cells in a serum free condition. This culture system is thus useful to study the effects of activin on prolactin production.(Kitakanto Med J 2006;56:1~9)

The Stimulatory Effect of Activin on PRL Production in GH3 Cells in a Serum-Free Culture