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Radiolabeled products were formed from labeled substrates during anaerobic incubation of sediments from Sites 618, 619, and 622. One set of experiments formed CO2, CH4, and SH2 from 2C-acetate and S-sulfate; a second set formed CH4 from C-methylamine or C-trimethylamine. Levels of CO2 and S~ formed were two to three orders of magnitude greater than CH4. Production of CH4 by Deep Sea Drilling Project (DSDP) sediments was four to five orders of magnitude less than that formed by anoxic San Francisco Bay sediment. However, incubation of Site 622 sediment slurries under H2 demonstrated production of small quantities of CH4. These results indicate that DSDP sediments recovered from 4 to 167 m sub-bottom (age 85,000-110,000 yr.) harbor potential microbial activity which includes sulfate reducers and methanogens. Analysis of pore waters from these DSDP sites indicates that bacterial substrates (acetate, methylated amines) were present.

Evidence for Sulfate-Reducing and Methane-Producing Microorganisms in Sediments from Sites 618, 619, and 622