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A section near the middle of core 10 from Site 147B collected at an approximate depth of 80 meters below the sediment surface, was freeze dried. A weight loss of 35.2 percent occurred. A 500 gram sample of the dried sediment was extracted twice with benzene-methanol mixture in an ultrasonic extractor. The extracts were combined and evaporated in a rotating, vacuum evaporator. They accounted for 0.10 weight percent of the starting,-dried sediment. The residue was then extracted with dilute sodium hydroxide and a humic acid fraction isolated and purified. It represented only 0.0034 percent. The residue was then neutralized, freeze-dried and split into two samples. The first was pyrolyzed overnight in a flowing stream of hydrogen at a pressure of one atmosphere and a temperature of 35O°C. The pyrolysate was collected in a trap chilled with dry ice. The residue in the pyrolysis tube was extracted with benzene-methanol and combined with the material in the trap. The weight of the benzene-soluble material after pyrolysis was 0.10 percent of the dried sediment. The second half of the extracted sediment was suspended in two liters of six normal sulfuric acid. Excess potassium dichromate was added and 500 milliliters of distillate was steam distilled from the refluxing reaction mixture. The distillate and the residue after oxidation were extracted separately with diethyl ether in a liquid-liquid extractor to obtain volatile and non-volatile acid fractions.

Characterization of the Organic Matter in a Site 147 Core from the Cariaco Trench