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The anisotropy complex refractive index of tissue is an important parameter in understanding the behavior of light, including its transportation in and interaction with tissues. We used the specular reflection method to investigate the anisotropy complex refractive index of chicken tissue with fibrous structures in vitro at a wavelength of 650 nm. The measurement data were highly consistent with the Fesnell equations. The results showed that the real refractive index was higher along the orientation of the fibers than along the cross section, but the imaginary refractive index was nearly identical. Furthermore, the fiber orientation was in the direction of the optic axis of the chicken tissue and the chicken tissue section was similar to a negative uniaxial crystal wafer.

Determination of the anisotropy complex refractive indices of chicken tissues in vitro at 650 nm