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This study was conducted for microbial hydrogen production from food waste and sewage sludge. Thirty three batch tests with different VS concentration (from 0.5 to 5.0 %, w/v) and mixing ratio of food waste to sewage sludge (from 0:100 to 100:0) were performed at 35°C. Heat-treated anaerobic sludge was used to seed the serum bottles. In all the tests, cumulative hydrogen production reached the maximum values within 2.5 days. n-Butyrate was produced simultaneously with hydrogen production, of which the amount was proportional to that of n-butyrate. Clostridium sp. are, therefore, considered to be the dominant microorganisms in this study because these microorganisms are responsible for n-butyrate fermentation. The hydrogen production potential of food waste was found over 34.0 mL/g VS at all the VS concentration. The maximum potential of 59.2 mL/g VS was found at 3.0 % of VS concentration. The potential decreased as sewage sludge composition increased due to the methanogens contained in sewage sludge and low carbohydrate concentration; however, the addition of sewage sludge to food waste enhanced hydrogen yield because of sufficient protein. The maximum hydrogen yield of 1.01 mole H2/mole hexoseadded was achieved at the food waste to sewage sludge ratio of 80:20 at the VS concentration of 3.0 %. The specific hydrogen production rate increased up to 22.6 mL H2/g VSS/h as both food waste composition and VS concentration increased.

Characteristics of hydrogen production from food waste and waste activated sludge