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In 2016, only 56.7% of the lakes and reservoirs in Japan complied with the standard of chemical oxygen demand determined by permanganate (CODMn). The dissolved organic matter (DOM) contributing to CODMn in lakes is heterogenous in nature; hence, DOM compositions in different lake waters are not well known. In this study, molecular components contributing to dissolved CODMn (DCODMn) in lake water were identified via high-resolution Orbitrap mass spectrometry (Orbitrap MS). Water samples were collected from Lake Inba in September 2016. The filtered samples were oxidized by KMnO4 according to the JIS method K0102 for different durations (ranging between 0–120 min). The DOM extracted via solid phase extraction was analyzed using Orbitrap MS, and molecular formulas were assigned. In all, 1,115 components were observed before oxidation. During time-course oxidation, components with higher unsaturation were selectively oxidized, suggesting that they contributed to the DCODMn in lake water. DCODMn components were screened for DOM in Lake Inba, and other DOM references were isolated from the Suwannee River and Lake Biwa. Only 14%–32% of the total components were regarded as DCODMn components, indicating the limited coverage of CODMn. The compositions of DCODMn components were highly diverse, depending on the sources.

journal of water and environment technology

Molecular-Level Changes in Dissolved Organic Matter Compositions in Lake Inba Water during KMnO&lt;sub&gt;4&lt;/sub&gt; Oxidation: Assessment by Orbitrap Mass Spectrometry