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The present investigation deals with pollen morphological study of flowering plants from Ramshej Fort, Mhasrul, Nasik, Maharashtra during the period of January 2016 to December 2016. Fresh flowers from diffe rent plants collected early in the morning before anthesis. Collected pollen grains treated by acetolysis technique and obse rved under light microscope for pollen morphological studies.The purpose of this survey work was to collect the pollen materials from different plants growing in the field and study of diffe rent pollen parameters such as shape , size, colpi and exine ornamentation. Pollen grain from plants such as Parthenium hysterophorous ,Alternanthera cicilis Hibiscus panduriformis ,Plumbago zelanica ,Malvastrum spp. ,Lantana camera ,Argyreaya species ,Cassia occidentalis, Brassica campestris ,Laiunea sarmenosa Cordiospermum heicabum ,Merremia species ,Vernonia cineraria ,Tridax procumbens ,Zenia pinnata ,Smithea spp. ,Ribulus tenestris, Desmodium scopiurus ,Macroptilium uniflorum ,Euphorbia geniculata, Ageratum conyzoides ,Peristrophe paniculata ,Borrahavia diffusa ,Ipomea marginata , Sonchus oleraceus ,Oxalis coniculata , Synedrela nudiflora ,Euphorbia hirta ,Cantarium meyeri were collected. Total 30 types of pollen grains from diffe rent plants collected. Out of these pollen types 7 be longing to Aste raceae, 3 from Malvaceae , 3 from Convolvulaceae, 3 from Fabaceae, 2 from Euphorbiaceae and one from Amaranthaceae, Plumbaginaceae, Verbenaceae, Caesalpinaceae, Brassicaceae, Sapinadaceae , Zygophylacaeae, Boraginaceae, Acanthaceae, Steraceae, Oxalidaceae and Gentiaceae family respectively. Among the collected taxa 9 shows trizonocolporate type, 4 shows polyzono he terocolporate type and one exhibits te trazonocolporate type of pollen grains. Te trazonocolporate type of pollen grain is found in Argyreya species. On the basis of pollen paramete rs the present investigated taxa like Parthenium hysterophorus, Plumbago, Lantana camara, Argyra spp., Cassia occidentalis and Brassica campestris having tricolporate pollen grains may be considered as in advanced status whereas Acacia auriculiformis has primitive status in scale of e volution. The present study is useful in the preparation of a comple te pollen calendar in different seasons of the area under investigation. The colporate structures could be noticed very clearly in acetolysed pollen grains. The prepared pollen slides of the taxa investigated may be used as refe rence slides for identifying the pollen grains captured from air.

LIGHT MICROSCOPIC STUDIES OF POLLEN GRAINS BY ACETOLYSIS METHOD