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Background:Today, cannabis sativa L. is the most consuming drug trafficking plant in the world after nicotine and alcohol.Aims:Given the properties of cannabis and psychoactive compounds of this opiate drug, including hashish, as well as the importance of human adipose-derived mesenchymal stem cells in the restoration and regeneration of organs and its application in tissue transplantation and tissue engineering, the current paper investigates the effect of hashish (hydroalcoholic extract of cannabis) on the morphology and growth process of human adiposed-drived mesenchymal stem cells in vitro.Methods:Human adipose-drived mesenchymal stem cells were extracted from the abdominal fat by the enzyme digestion of the patient’s abdominal region. MTT test was performed to determine the best concentration of cannabis extract. Cells were treated for seven days at 100 ng / ml and 1000 ng / ml, and propagation, growth and viability of the cells were evaluated.Results:The results show that cannabis extract reduces the proliferation of human adipose-drived mesenchymal stem cells as time-dependent. Also, the effect of cannabis extract on mesenchymal cells depends on concentrations. High concentrations have a greater negative effect on the growth of the cells. The results of this study showed that the morphology of human adipose-drived mesenchymal stem cells did not change after exposure to cannabis extract.Conclusion:Although in the first days, the growth process is elevated and the cells are in the euphoric phase, apoptosis increases with time as consumption increases due to toxicity. The effects of cannabis on fat cells indicate a decrease in the volume of adipose tissue and, consequently, in the consumer’s slimming.

Effect of hydroalcoholic extract of Cannabis (Cannabis sativa L.) on morphology and the process of human adipose-drived mesenchymal stem cell growth