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The application of ultrasound in mice has increased the activity of mice and showed anxious behavior that affects the blood cells of mice. This study aims to observe the effect of 40 kHz ultrasound exposure on the content of leukocytes and erythrocytes in blood cells of mice. For this purpose, five ultrasound treatments with sound pressure levels were 70, 75, 80, 85, and 90 dB respectively and one control in six sample groups, each consisting of five mice with the same weight and age. Sound treatment is carried out with a 40 kHz continuous ultrasound exposure of the same duration for two hours. Taking blood samples of mice is carried out as soon as the sample is given a sound treatment. Based on the results of blood tests of mice it was found that the average increase in the number of leukocyte mice varied between 40-1,070 cells/mm3 for variations in sound pressure levels from 70 to 90 dB with an increase in the highest average number of leukocyte (1,070 cells/mm3) at the sound pressure level 75 dB. With the same level of sound pressure level, the decrease in the average number of erythrocytes varied between 920,000-190,000 cells/mm3 with a decrease in the highest average number of erythrocytes (920,000 cells/mm3) at an 80 dB sound pressure level. The optimum level of sound pressure that can affect the average number of leukocytes and erythrocytes in blood cells of mice are still unknown.

Treatment of 40 kHz continuous ultrasound towards blood cells of mice (Mus musculus L)