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Over the past few decades biotechnology field related to design of nutrient media containing animal and plant protein enzyme hydrolysates as amino acid sources has been established and developed [5, 7]. It was established that 13 amino acids are needed for vertebrate cell growth outside the organism (isoleucine, leucine, lysine, methionine, phenylalanine, threonine, triptophane, valine, arginine, glutamine, histidine, tyrosine, cysteine) [2, 10]. Protein hydrolysates are the products of protein cleavage in amino acids and simple peptides. Hydrolysates usually consist of amino acids with variable concentration as well as peptides of different molar weight [4, 8]. Blood protein hydrolysate (BPH) contains the sufficient amount of amino acids necessary for BHK-21/2-17 cell growth [6]. All amino acids participate in biosynthesis of different proteins. Amino acid performance of this or that function in vitro mostly depends on conditions of cell cultivation. One of the reasons for presence of amino acids not needed for the whole organism in the medium is their restricted synthesis in the culture [4, 11] Some authors mark that the demand in these organic acids depends on different types of cells. Very often amino acids are added to compensate the inability of some cells to synthesize them or to compensate their leakage into the medium [13]. SUMMARY The raw material for blood protein hydrolysate preparation is whole animal blood, its clots and other serum production wastes. The dependence of ВНК-21/2-17 cell lineage productivity on the season of hydrolysate raw material preparation was observed. Cell growth stimulated by blood protein hydrolysate from raw material prepared in summer months 1.2–1.3 times exceeded cell growth stimulated by blood protein hydrolysate prepared in autumn-winter period (differences are significant, p < 0,001). The amount of FMDV immunogenic components from “summer” blood protein hydrolysate 1.3–1.6 exceeded the amount obtained during other seasons of the year (differences are significant, p < 0,001). There was found a direct correlation between BHK-21/2-17 cell lineage productivity, concentration of FMDV immunogenic components and seasonal dynamics of the contents of the following amino acids composing blood protein hydrolysates: alanin, asparagine, valine, histidine, glycine, isoleucine, leucine, lysine, methionine, proline, serine, tyrosine, threonine, tryptophane, and phenylalanine. To increase BHK-21/2-17 cell lineage productivity and concentration of FMDV immunogenic components it was suggested to add some additional amino acids into the culture growth medium for suspension cell culture in autumn-summer period together with blood protein hydrolysate.

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INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS