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espanolActualmente, el uso de bacterias promotoras de crecimiento vegetal resulta una alternativa viable al uso indiscriminado de agroquimicos. Con el fin de obtener bacterias con capacidad de promocion del crecimiento vegetal, en este trabajo se aislaron doce cepas con actividad de fijacion de nitrogeno a partir de la rizosfera de zacate bermuda (Cyonodon dactylon) ruderal. Con base en la rapidez e intensidad del cambio de color en la prueba cualitativa de fijacion del nitrogeno, se seleccionaron dos aislados para su identificacion molecular y caracterizacion. La amplificacion, secuenciacion y analisis de fragmentos del gen ribosomal 16S rRNA permitio identificar a Pseudomonas monteilii (Pm0710) y Chryseobacterium massiliae ca (Cm0711). Ambos aislados mostraron actividad de solubilizacion de fosfatos, de produccion de acido indol-3-acetico, acido giberelico y sideroforos, de fosfatasa alcalina y ACC deaminasa, asi como de inhibicion contra: Fusarium brachygibbosum, F. falciforme, F. oxysporum y Ceratobasidium sp. Aunque Cm0711 fue significativamente superior que Pm0710 en la mayoria de las propiedades analizadas, las dos bacterias tienen potencial para ser utilizadas como bioinoculantes; sin embargo, antes se debe realizar su validacion in vivo. Palabras clave: Ceratobasidium, Chryseobacterium massiliae, Fusarium, Pseudomonas monteilii, PGPR EnglishCurrently, the use of plant growth promoting bacteria is a viable alternative to the indiscriminate use of agrochemicals. In order to obtain bacteria with the capacity to promote plant growth, in this work twelve strains with nitrogen fixation activity were isolated from the rhizosphere of bermuda grass (Cyonodon dactylon) ruderal. Based on the speed and intensity of the color change in the qualitative nitrogen fixation test, two isolates were selected for molecular identification and characterization. The amplification, sequencing and analysis of 16S rRNA ribosomal gene fragments allowed to identify Pseudomonas monteilii (Pm0710) and Chryseobacterium massiliae ca (Cm0711). Both isolates showed phosphate solubilization activity, production of indole-3-acetic acid, gibberellic acid and siderophores, alkaline phosphatase and ACC deaminase, as well as inhibition against: Fusarium brachygibbosum, F. falciforme, F. oxysporum and Ceratobasidium sp. Although Cm0711 was significantly higher than Pm0710 in most of the properties analyzed, the two bacteria have potential to be used as bio-inoculants; however, before validation must be done in vivo. Keywords: Ceratobasidium, Chryseobacterium massiliae, Fusarium, Pseudomonas monteilii, PGPR

Actividad in vitro de promoción del crecimiento vegetal y control biológico de rizobacterias aisladas de zacate bermuda ruderal