English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Pro

Presents the zaia usage as premium feature

ZAIA

Presents AI Discovery search usage as a premium feature

AI Discovery

Presents AI Discovery explain and summary usage as a premium feature

Content Analysis

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Presents the pdf analysis as premium feature

PDF Analysis

1500 Top-up

3000 Top-up

Zendy Basic

2500 Top-up

Zendy Unlimited

Presents the access of premium content as premium feature

Premium Content

Received: Revised: Accepted: Published online: April 12, 2018 July 02, 2018 July 03, 2018 September 04, 2018 Avian influenza virus (AIV) and Newcastle disease virus (NDV) are systemic disease that is highly infectious with high morbidity and mortality rates, and highly detrimental to economic value in the domestic and commercial poultry industries. Generally, in the field there is a mixed infection of AIV and NDV, each of which is highly pathogenic. Clinical signs are difficult to distinguish, so immunopathological immunohistochemistry (IHC) double staining was developed with the ability to confirm the diagnosis of mixed infection. In the present study, 20 chickens infected naturally with clinical signs of torticollis and curled toe paralysis that had a mixture of AIV and NDV infections and there are variations of tropism distribution of AIV and NDV in tissues were used. The AIV and NDV were visualized by performing the double staining technique DAB and Fast-Red with monoclonal antibody anti-hemagglutinin AIV-mouse (AIVHA) and polyclonal antibody antihemagglutinin-neuraminidase NDV-rabbit (NDVHN) proteins. AIV infected-lungs or brains had red coloration (AIVHA) and NDV infected lungs or brains had brown coloration (NDVHN). The IHC double staining technique is sensitive and accurate in the confirmation of the diagnosis of a mixed infection to determine the difference between AIV and NDV. ©2018 PVJ. All rights reserved

pakistan veterinary journal

Detection of Mixed Infection of Avian Influenza and Newcastle Disease Viruses in Chickens in Indonesia by Immunopathologic Immunohistochemistry Double Staining