ROLE OF THE IL-6 / TNF RATIO IN DIAGNOSTICS OF PERINATAL INFECTIONS

Sporadic miscarriage is mostly associated with infectious causes. Infectious agents on the background of the gestational immunomodulation realize the pathogenic effect by direct damaging effect and through the cytokine system. In this case, it is relevant to study the functioning of the immune system in pregnant women. Given the interaction of cytokines in the development of the inflammatory reaction and the wide variability of their concentrations in the venous and umbilical cord blood of patients. Ratio of IL-6 to TNFα (IL-6v / TNFαv) in venous blood for infection-related gestational pathology and similar coefficient for cord blood cytokines was studied. Materials and Methods. The case group has consisted of patients with diagnosis of spontaneous preterm birth (before 37 weeks’ gestation). Healthy women with the term labor (≥37weeks) were included into the control group. All participants were at least 18 years old at enrollment. All patients in the preterm group had spontaneous delivery between 27+1 and 36 + 6 weeks of gestation. Maternal plasma levels of IL-6 and TNFα different stages of pregnancy were quantified with enzyme linked immunosorbent assay. Mann-Whitney U test was used to compare two independent groups. Odds ratio events in the one group to the chances of the same event in another (OR) and 95% confidence interval for them (95% CI) were calculated. Method of constructing a ROC-curves with the calculation of the area under the curve (AUC), sensitivity (Se) and specificity (Sp) obtained models were used for diagnostically significant indicators of quantitative traits. Significance was established as p < 0.05. Statistical analysis was performed using the program «MedCalc 10.2.0.0» (MedCalc, Mariakerke, Belgium). Results. Ratio of IL-6 / TNFa in the venous and umbilical cord blood in preterm and term labor was as follows (table 1). Table 1. The ratio of IL-6 to TNF in venous and cord blood in preterm and term labor, n; Me (25; 75)