English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

It was first established that the risk of developing lung cancer in the Yakut population is associated with the zero genotype of the GSTM1 gene, which may indicate a higher risk of cancer for carriers of this genotype. Polymorphism in the genes GSTM1, GSTT1, which encodes the isoforms μ, t of the glutathione-S-transferase enzyme characterized by deletion in both alleles leads to complete absence of synthesis of the protein product and resulting in a deep suppression of the enzyme function. According to the reports, there is conflicting data on the association of deletiongenotype GSTM1 with the risk of developing lung cancer. [4] The aim of the research was to study polymorphism of the GSTT and GSTM1 genes patients with lung cancer. Materials and Methods. Molecular genetic studies were conducted in 36 people of Yakut nationality with lung cancer. The control group was selected taking age and ethnicity into account. It included 65 people. The main criteria for selecting a control group were the absence of cancer. A standard phenolic-chloroform extraction method was used to isolate DNA. The analysis of polymorphic variants of specific sections of the genes GSTM1, GSTT1 was carried out using polymerase chain reaction methods and while using the primer structure described in the works (Gronau et al., 2003). When comparing genotype frequencies, the standard χ2 criterion was used. The relative risk (OR) of the development of the disease with a specific genotype was calculated using the standard formula OR = a / bxd / c, where a and b are the number of patients with and without a mutant genotype, respectively, and d, c is the number of people in the control group who have and don’t have a mutant genotype. OR is indicated with a 95% confidence range. Results. According to our data in the selection studies of patients with lung cancer and control it is revealed that the frequency of occurrence of the “zero” genotype GSTM1 (0/0) in the group of patients was higher (66.6%) than in healthy individuals (43.0%). Where differences has reached a level of statistical significance χ2 = 5.16, p = 0.023, OR = 0.67 (0.26-1.69). The analysis of deletion polymorphism of the GSTT1 gene did not reveal any significant differences in the frequency distribution of genotypes depending on oncopathology. The frequency of occurrence of deletion polymorphism in the group of patients was 41.7%, and in the healthy group 32.3% (χ2 = 0.88, p = 0.347, OR = 0.61 (0.26-1.69)). Obviously, the GST genes, especially GSTM1, are involved in the pathogenesis of various types of cancer and act as modifiers and risk factors for a variety of diseases associated with adverse environmental factors. According to published data of other authors, the influence of polymorphism of GST-genes is associated with the development of numerous malignant tumors: lung cancer [6], bladder, rectum, stomach, esophagus, breast, ovaries and skin [1,2,3,5,7,9]. There are works showing the connection of polymorphism of genes encoding the enzymes of the second phase of detoxification, with chronic and hereditary diseases: chronic bronchitis, bronchial asthma [8]. In addition, it has been shown that the deletion of the GSTM1 and GSTT1 genes leads to increased sensitivity to xenobiotics (in particular, to thermal decomposition products of tobacco). In our work with a high degree of reliability it was shown that the increased risk of developing lung cancer in the Yakut population can be related to the zero genotype of the GSMT1 gene. Prospects for further research. It was found that the risk of developing lung cancer in the Yakut population is associated with the zero genotype of the GSTM1 gene, which may indicate a higher risk of cancer for carriers of this genotype. This can cause a severe tolerance of chemotherapy treatment of Yakut patients with lung cancer. Studies in this direction can be continued. This could be a study not only of polymorphism of the genes GSTM1 and GSTT1 but also of other polymorphisms of xenobiotic detoxification genes in Yakut population.

POLYMORPHISM OF BIOTRANSFORMATION GENES OF XENOBIOTICS GSTM1 AND GSTT1 OF PROBABLE MARKERS OF RISK OF LUNG ONCOLOGY, IN THE YAKUT POPULATION