English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The secretion of extracellular vesicles (EVs) has been neglected in Gram-positive bacteria due to the absence of an outer membrane and the difficulties of proper visualization. Here we aimed to prove that lactobacillus casei can secrete extracellular vesicles. Methods: EVs were extracted from Lactobacillus casei, cultured in De Man, Rogosa and Sharpe broth, using a polyethylene glycol (PEG) solution. The characteristics of the EVs were analyzed by electron microscopy, Dynamic Light Scattering (DLS) and SDS-PAGE. Results: The electron microscopy showed rounded vesicles with average diameter of 300 nm. The protein content of this nanostructure was 2.5 mg/ml with a protein pattern within the range of 10-200 kDa. DLS result showed populations of approximately 300 nm while the extracted EVs had a negative zeta potential. Conclusion: A new method of producing functional molecules from probiotic bacteria was presented. Our results indicated EVs purity with acceptable conformation. Further investigations are necessary to elucidate the efficacy, practicality and mechanism of action of such EVs in clinical practices, especially for development of bio-compounds and vaccine delivery vehicles.

A polyethylene glycol-based method for extraction of extracellular vesicles from Lactobacillus casei as vaccine delivery vehicle