English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Dendritic cells (DCs) have been recently employed as carriers for vaccines against several viral infections. The present study was designed to develop a prophylactic vaccine against hepatitis C virus (HCV) using DCs treated with Berberis vulgaris root extract (BRE), as a preclinical study. Methods: BRE was prepared and injected to female BALB/c mice for DCs expansion. The expanded splenocyte cells (EDC) were purified and efficiently loaded ex vivo with HCV-nonstructural protein 3, NS3, (EDC-NS3). Mice were subcutaneously inoculated with EDC-NS3 vaccine candidate thrice with 4-week-intervals and IL-12, IFN-γ, IL-4, IL-10, MHC class II, CD3, CD16, indoleamine 2, 3 dioxygenase (IDO) and total protein levels were measured, post-vaccination by PCR and flow cytometry. Moreover, cytotoxic T lymphocyte and humoral immune responses were examined. Results: Our data revealed that immunization with EDC-NS3 vaccine elevated IL-12, IFN-γ and IL-4 expressions as well as MHC II and CD16 at protein levels. It also elicited strong HCV-NS3-specific humoral and cellular immune responses. However, the expressions of CD3, IDO, and IL-10 were down-regulated, post-vaccination. Conclusion: EDC-NS3 immunization serves as an innovative modality for immunoprophylaxis against HCV infection.

vaccine research

Constructions of hepatitis C Virus prophylactic vaccine candidate using Berberis vulgaris stimulated and nonstructural protein 3 loaded dendritic cells