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As you know the particle size is the most important issue in preparation of nanoparticles. Nowadays, conventinal drug therapy is characterized by limited effectiveness and undesirable side effects which are due to lack of selectivity and poor distribution. Hence, reduction of particle size is considered as an approach to reduce toxicity, increase release and bioavailability, improve solubility and produce a better formulation for a higher quality. Therefore, reduction of the particle size of nanoparticles is important. We would like to present a new method we used to reduce the particle average size to 64 nm. In the study of Sankar et al. an aqueous extract was used to prepare the nanoparticle and the size of the titanium dioxide nanoparticle synthesized with aqueous Origanum vulgare extract in a Dynamic Light Scattering Analysis was 341 nm. We used hydroalcoholic extract and prepared titanium dioxide nanoparticles by using Origanum vulgare hydroalcoholic extract. The size of nanoparticles we prepared were much smaller average size (64 nm), which are about one-fifth of the nanoparticle sizes produced in Renu Sankar et al. study. Core tip  *Correspondence to Dr. Mahmoud Bahmani mahmood.bahmani@gmail.co m

A new method for promoting biologic synthesis and reducing the size of titanium dioxide nanoparticles (Tio2 NPs) synthesized by Origanum vulgare